For Cx36, the staining intensity was higher in the CA2 region in the epilepsy group. There was, however, regional variation within each Cx subtype. There was no evidence of any differential expression of Cx36. Two independent reviewers rated each Cx–region combination according to an immunoreactive score.Īcross all three measures-that is, staining intensity, percentage of positively stained cells, and immunoreactive score-Cx32 appeared to be expressed at a significantly lower level in the epileptic patients compared with controls (p < 0.001 for each measure), whereas Cx43 appeared to be expressed more among the epileptic patients (p < 0.001 for each measure). To state that gap junctions synchronize neuronal activity and that this translates into seizure facilitation belies the complexity of the brain and the. Each slide demonstrated multiple cells from each of six regions (CA1, CA2, CA3, CA4, dentate gyrus, and subiculum). Immunostaining was performed to create one slide for each of the three Cxs. These expression levels were compared with those in hippocampi obtained in nonepileptic patients during postmortem dissection. However alluring is the hypothesis linking neuronal gap junctions to epileptic seizures, there remain several caveats. ![]() The expression of Cxs32, -36, and -43 was studied in 47 consecutive samples of hippocampi obtained from epileptic patients who had undergone an amygdalohippocampectomy for the treatment of intractable seizure.
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